AnnoAudit: a marker-based protocol for auditing single-cell atlas annotations reveals annotation-driven artifacts in a widely used traumatic brain injury resource
Single-cell atlas annotations are routinely treated as ground truth but rarely validated before use. We present AnnoAudit, a marker-based audit protocol that combines four convergent checks - marker scoring, unsupervised clustering, margin-gated module scoring, and applicability-gated pretrained models - into a composite Annotation Contamination Score (ACS), plus a trajectory-correlation…
We haven't written up this one. bioRxiv has the full story — the link below goes straight to it.
