Differential LncRNA expression profile in the plasma of preeclampsia and normal pregnancies
Background: Early detection of preeclampsia with noninvasive and reliable biomarkers is the foremost step for minimizing adverse effects during pregnancy. However, none maternal serum analyte evaluated so far is sufficiently accurate to allow recommending their routine use. Methods: Microarray was used to first identify differentially expressed LncRNA and mRNA. Gene ontology (GO) and the Kyoto…
An early detection method using noninvasive and reliable biomarkers is crucial for minimizing adverse effects in pregnant women with preeclampsia. However, none of the maternal serum analytes currently evaluated have proven to be accurate enough for routine use.
Researchers employed microarray technology to identify differentially expressed Long Non-Coding RNAs (lncRNAs) and messenger RNAs (mRNAs). Subsequent gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed the biological functions and signaling pathways associated with these genes. To further explore the interaction of these genes, a quantitative real-time polymerase chain reaction was used to validate the microarray results, and a lncRNA-mRNA co-expression network was constructed.
The study found that 1476 lncRNAs and 594 mRNAs were differentially expressed in the plasma of patients with preeclampsia when compared to normal pregnancies. Interestingly, T241171, T338586, and uc002ywy.3 were up-regulated while ENST00000524858, T131416, T357032, and uc.335+ were down-regulated. The pathway analysis indicated that the targeted mRNAs were enriched in apoptosis, sulfur metabolism, and starch and sucrose metabolism.
Furthermore, the researchers constructed a ceRNA (competitive endogenous RNA) network, which suggested that lncRNA uc002ywy.3 could be an upstream regulator of miRNA-4498. This specific lncRNA-miRNA pair was predicted to interact with genes involved in the PD-1/PD-L1 pathway, according to KEGG analysis. The conclusion drawn from this study is that the PD-1/PD-L1 signaling pathway may play a role in the development of preeclampsia.
Moreover, the dysregulated LncRNA uc002ywy.3/miRNA-4498 pair highlights a new layer in the regulatory network of preeclampsia.
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