VLCFA-mediated inter-cell layer communication controls cellular pluripotency in Arabidopsis callus
Plants have remarkable capacity to reconstruct entire organ systems from tissue explants. In Arabidopsis two-step tissue culture system, pluripotency regulators are specifically expressed in the middle-cell layer of the stratified callus tissue. However, regulatory mechanisms underlying the radial patterning of callus remained unclear. Here, we found that very-long-chain fatty acids (VLCFAs)…
Arabidopsis plants possess the unique ability to regenerate entire organ systems from tissue explants. In a two-step tissue culture system, pluripotency regulators are expressed in the middle-cell layer of the stratified callus tissue. However, the mechanisms behind radial patterning of the callus were not well understood.
This study reveals that very-long-chain fatty acids (VLCFAs) synthesized in the epidermis-like outermost layer are crucial for pluripotency acquisition and successful shoot regeneration. Through genetic and transcriptomic analyses, researchers discovered that VLCFAs exert their regulatory roles on pluripotency acquisition via inter-cell layer signaling within the callus tissue.
Although VLCFAs are not dependent on ATML1/PDF2 functions or cuticular wax synthesis in the outermost layer, they play a role in spatially restricting procambium cell identity.
By non-cell-autonomously suppressing cytokinin signaling, VLCFAs enable the establishment of the middle-cell layer. This inhibitory relationship between layer-specific regulators maintains the delicate balance required for cellular fate determination in pluripotent callus.
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