Feasibility of adopting water displacement and 3D scanning methods to quantify lung volume reduction in ex vivo models
Lung volume reduction is a treatment for chronic obstructive pulmonary disease (COPD) patients with severe emphysema. Currently, in vivo animal models are used as a first step to quantify the efficiency of novel lung volume reduction treatments. By quantifying lung volume reduction in ex vivo samples, animal testing may be reduced. This study proposes two new methods for quantifying lung volume…
Lung volume reduction serves as a treatment for COPD patients suffering from severe emphysema. At present, animal models are utilized to assess the effectiveness of new treatments that target lung volume reduction. However, employing ex vivo samples could potentially decrease the reliance on animal testing. This research introduces two novel methods for determining lung volume reduction in ex vivo tissues: water displacement and three-dimensional (3D) scanning.
Porcine lung lobes were inflated and coated with cyanoacrylate glue to mimic lung volume reduction. Volume alterations were assessed using a bespoke water displacement apparatus and in a ventilated environment utilizing a handheld Artec Space Spider 3D surface scanner. Both approaches identified post-treatment volume reductions.
Water displacement offers a straightforward and affordable solution for isolated lung lobes, whereas 3D scanning facilitates volumetric analysis of complete lungs under ventilated circumstances. These techniques present a dependable intermediate stage between in vitro and in vivo testing, ultimately leading to a reduction in animal usage and an enhancement in the preclinical evaluation of novel lung volume-altering interventions.
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