IKK2/β mediated phosphorylation of transcription factor Ets2 at site(s) distal to DNA binding domain negatively modulates its DNA binding activity
Transcription factor Ets2 coalesce with the NF-{kappa}B pathway to regulate gene expression in specific signaling contexts. IKK2/{beta}-mediated phosphorylation events critically regulate the NF-{kappa}B pathway. However, any link between Ets2 and IKK2 remains elusive. Here we report Ets2 as a direct substrate of IKK2. In-vitro kinase assays using deletion constructs, high resolution MS-MS and…
A new study has uncovered a previously unknown connection between the transcription factor Ets2 and the IKK2/β signaling pathway. Researchers found that Ets2 can be directly phosphorylated by IKK2, a critical regulator of the NF-κB pathway. Phosphorylation of a specific site, S295, located distal to the DNA binding domain, appears to negatively impact Ets2's ability to bind to DNA.
This finding was confirmed through various experimental techniques, including MD simulations and electrophoretic mobility shift assays. The researchers' work sheds light on an unexpected regulatory link between Ets2 and IKK2, with implications for understanding how these two molecular players interact in different signaling contexts.
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