Bacterial metagenome in plaque, saliva, and tumor samples from individuals with and without OSCC by next-generation sequencing
Background: Oral dysbiosis has been associated with oral squamous cell carcinoma (OSCC); however, most microbiome studies rely on 16S ribosomal RNA (rRNA) gene sequencing, limiting species-level taxonomic resolution. Methods: Dental plaque, saliva, and tumor tissue samples from 10 patients with OSCC and dental plaque and saliva samples from 10 healthy controls were analyzed in this exploratory…
Dental plaque, saliva, and tumor tissue samples from patients with and without oral squamous cell carcinoma (OSCC) were subjected to shotgun metagenomic sequencing in an exploratory study. This method, using the Illumina MiSeq platform, allowed for higher taxonomic resolution compared to previous 16S rRNA gene sequencing studies.
After quality filtering and taxonomic classification, comparative analysis revealed distinct bacterial community profiles across the oral microenvironments of OSCC patients and healthy controls. Dental plaque showed the greatest diversity and abundance, with an enriched presence of certain bacterial species in OSCC patients compared to the controls.
Saliva samples, while less diverse, still harbored specific bacterial strains in higher relative abundance among OSCC patients. Tumor tissue exhibited a relative increase in certain bacterial species as well. These findings provide a species-level characterization of the oral microbiome in OSCC, suggesting potential targets for future integrative studies.
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