Proteomic Profiling of Enriched Nuclei Provides a Nuclear Proteome Resource and Protein Interaction Landscape for Trypanosoma cruzi
The processes such as DNA replication, transcription, and repair are often modulated by specific nuclear proteins, protein-protein interactions (PPIs), and post-translational modifications (PTMs). In Trypanosoma cruzi, however, the nuclear proteome and interactome have not been systematically mapped, limiting the interpretation of nuclear regulatory processes. Here, we report a nuclear proteome…
Research has identified a complete nuclear proteome and interaction landscape for the protozoan parasite Trypanosoma cruzi. Proteomic profiling of intact nuclei, through high-resolution mass spectrometry, revealed 1,734 proteins, 316 of which contained post-translational modifications. Computational analysis, using the STRINGDB database, organized the proteins into functional clusters, including a neighborhood of histone-associated interactions.
Crosslinking mass spectrometry further detected 26 residue-resolved protein-protein crosslinks, involving 36 proteins, and identified post-translational modifications at or near the linked residues. This comprehensive resource paves the way for comparative nuclear proteomics studies, multi-omics integration, and the prioritization of potential targets for functional investigations.
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