Multi-color droplet digital PCR assay enables allele-specific quantification of heterogeneous genome editing outcomes
Precise characterization of genome editing outcomes remains a major challenge because edited cell populations contain diverse alleles generated by homology-directed repair, non-homologous end joining (NHEJ), or base editing. While next-generation sequencing enables comprehensive analysis, its routine use is constrained by cost and turnaround time. Here, we developed a multi-color droplet digital…
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