A survey of multi-targeting and off-targeting sgRNAs across five genome-wide CRISPR-Cas9 knockout sgRNA libraries with GuideRefine
Pooled genome-wide CRISPR-Cas9 knockout (CRISPR-KO) screening is a powerful approach for discovering new biology and identifying genetic vulnerabilities in cancers. This approach uses the Cas9 nuclease in combination with sgRNA libraries, typically consisting of 4-8 sgRNAs to induce mutations in each target gene. A critical assumption is that the effect of each sgRNA is solely due to Cas9 editing…
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