Time-Resolved Phenotypic and Transcriptomic Responses of Primary Canine Dermal Fibroblasts to Prolonged Hypothermic Stress
Prolonged low-temperature exposure may extend the preservation window of mammalian cells but can also disrupt cellular homeostasis and ultimately compromise cell viability. This study investigated the time-dependent phenotypic and transcriptomic responses of primary canine dermal fibroblasts to sustained hypothermic stress. Passage-three fibroblasts were continuously maintained at 15 for up to 15…
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