A generalisable method for the purification and biophysical characterisation of bacterial membrane receptors
Membrane embedded bacterial receptors are challenging to express and purify in soluble form, yet their isolated domains are essential tools for structural and ligand discovery studies. Pseudomonas aeruginosa relies on the TonB dependent heme receptor HasR for iron acquisition, a process central to its pathogenicity. Here, we report a robust strategy for the recombinant expression, purification,…
We haven't written up this one. bioRxiv has the full story — the link below goes straight to it.
