Asynchronous structural rearrangements govern constriction of the human nuclear pore complex
The nuclear pore complex (NPC) is responsible for transport between the cytoplasm and nucleus. CryoET experiments have resolved multiple conformations of the pore that are dilated or constricted. Since the dilation state will impact transport properties, there is much interest in understanding the energetic factors that regulate dilation/constriction. To this end, we applied a structure-based…
The nuclear pore complex (NPC) serves as the conduit for transport between the cytoplasm and nucleus within human cells. CryoET experiments have unveiled various conformations of the pore, including both dilated and constricted states. The dilation state plays a crucial role in determining transport properties, making it essential to comprehend the energetic factors governing these changes.
Researchers applied a structure-based model akin to a game to investigate the mechanistic aspects of large-scale constriction, which can measure up to 100 Å, in the human NPC. Despite the symmetric nature of the structures, simulations demonstrated that the process involves asynchronous rearrangements. By exploring different versions of the model, scientists further elucidated the individual contributions of proteins to this collective motion.
This investigation offers a foundational understanding of the energetic and structural elements involved in NPC constriction, paving the way for the development of precise physical methods to study this intricate process.
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