Enhancing hypercompact Cas{Phi}2 activity through EPICA.2, an optimized eukaryotic directed evolution platform
Compact Cas nucleases offer advantages over the widely used SpCas9 due to their smaller size, which enables more efficient delivery for in vivo applications. Among these, the phage-encoded Cas{Phi}2 (Cas12j2) is highly promising due to its relaxed PAM requirement (5'-TTN-3') and compact size (757 aa); however, its translational potential is limited by low editing activity. To enhance the efficacy…
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