Mandatory use of mock communities highlighted by the descriptive comparison of Epi2Me 16S and EMU bioinformatic workflows for full-length 16S rRNA Nanopore sequencing.
Abstract Full-length 16S rRNA gene sequencing using Oxford Nanopore Technologies has emerged as a promising approach to improve species-level resolution in microbiota studies. However, the accuracy of taxonomic assignment remains highly dependent on the bioinformatics s used to process Nanopore long-read data. Therefore, the only way to ensure a good level of certainty in obtained results is to…
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