{
  "id": 6533280,
  "title": "mBACS enhances pseudouridine profiling through efficient and robust chemical conversion",
  "url": "https://urgent.news/2026/09/09/mbacs-enhances-pseudouridine-profiling-through-efficient-and-robust",
  "topic": "science",
  "section": "Science",
  "published": "2026-09-09T00:00:00.000Z",
  "source": {
    "name": "bioRxiv",
    "slug": "biorxiv",
    "url": "https://www.biorxiv.org/content/10.64898/2026.09.05.749584v1?rss=1"
  },
  "original_language": "en",
  "account": "Pseudouridine ({Psi}) is a widely occurring RNA modification that influences RNA stability, splicing, and translation. Researchers previously created 2-bromoacrylamide-assisted cyclization sequencing (BACS) to quantify single-base {Psi} detection accurately. In this study, scientists discovered methyl 2-bromoacrylate-assisted cyclization sequencing (mBACS), a new method that surpasses BACS in terms of {Psi} conversion efficiency and minimizes false-positive occurrences.\n\nThis advancement enabled mBACS to detect more sensitive and robust {Psi} sites within human tRNAs. Moreover, mBACS identified TRUB1 and PUS10 as the sole redundant writers of the conserved tRNA {Psi}55 modification, unveiling a connection between {Psi}55 and other tRNA modifications. Additionally, the study revealed 5-fluorouracil as a substance capable of inhibiting pseudouridine synthesis across various spots, causing a selective remodel of pseudouridylation across the genome.\n\nLastly, mBACS confirmed the feasibility of high-quality {Psi} profiling even from minute samples of RNA, measuring as little as 10 ng, thereby establishing mBACS as a highly sensitive, quantitative, and low-input second-generation platform for pseudouridine analysis.",
  "summary": "Pseudouridine ({Psi}) is the most abundant RNA modification and regulates RNA stability, splicing, and translation. Utilizing the dual nucleophilic N1 and O2 of {Psi}, we previously developed 2-bromoacrylamide-assisted cyclization sequencing (BACS) for quantitative, single-base {Psi} detection. Here, we screened new dual electrophiles and developed methyl 2-bromoacrylate-assisted cyclization…",
  "key_points": [],
  "editors_take": null,
  "illustration": null,
  "coverage": {
    "outlets": 1,
    "also_reported_by": []
  },
  "ai_generated": true,
  "disclaimer": "Summaries, key points and the editor’s take are written by software from other outlets’ reporting and may contain errors — always check the linked original."
}