{
  "id": 3230730,
  "title": "N-glycome analysis of dried blood spots from different blood preparations and its potential for pre-diabetes and diabetes distinction",
  "url": "https://urgent.news/2026/08/25/n-glycome-analysis-of-dried-blood-spots-from-different-blood",
  "topic": "health",
  "section": "Health & Medicine",
  "published": "2026-08-25T00:00:00.000Z",
  "source": {
    "name": "bioRxiv",
    "slug": "biorxiv",
    "url": "https://www.biorxiv.org/content/10.64898/2026.08.24.746065v1?rss=1"
  },
  "original_language": "en",
  "account": "Dried blood spot (DBS) sampling has gained popularity as a more convenient and safer alternative to traditional blood sampling methods. One promising application of DBS analysis is the examination of N-glycosylation, a post-translational modification of proteins linked to various biological and pathological functions. In this study, researchers employed a protocol involving ultra-high-performance liquid chromatography based on hydrophilic interactions and fluorescence detection (HILIC-UHPLC-FLR) to profile N-glycosylation in DBS. The protocol includes cutting the DBS, extracting the proteins, enzymatic digestion, labeling with 2-aminobenzamide, cleanup, and measurement using HILIC-UHPLC-FLR.\n\nThe researchers compared DBS N-glycosylation profiles with those of plasma and discovered that DBS maintain a stable N-glycosylation profile regardless of whether they are collected from fresh blood, frozen whole blood, or a combination of frozen blood cells and corresponding frozen plasma. Furthermore, the researchers examined DBS N-glycans from individuals with pre-diabetes and diabetes and found that fucosylation, bisection, and galactosylation showed a statistically non-significant increasing trend in diabetes, while sialylation demonstrated a statistically non-significant decreasing trend in diabetes. The primary advantage of this method is its ability to repurpose samples initially intended for other purposes, such as frozen whole blood. Moreover, DBS N-glycan profiling offers a simpler, more cost-effective, and less invasive approach to diagnosing and monitoring pre-diabetes and diabetes patients compared to conventional plasma analysis.",
  "summary": "Dried blood spot (DBS) sampling is becoming a popular alternative to traditional blood sampling approaches, offering advantages such as convenience of collection, transportation, and storage, as well as lower biohazard risk. N-glycosylation, a major post-translational modification of proteins associated with numerous biological and pathological functions, is one area of interest for DBS analysis.…",
  "key_points": [],
  "editors_take": null,
  "illustration": null,
  "coverage": {
    "outlets": 1,
    "also_reported_by": []
  },
  "ai_generated": true,
  "disclaimer": "Summaries, key points and the editor’s take are written by software from other outlets’ reporting and may contain errors — always check the linked original."
}