{
  "id": 3153860,
  "title": "Click-Prep: An Interactive Data Preparation Tool for Click-qPCR",
  "url": "https://urgent.news/2026/08/24/click-prep-an-interactive-data-preparation-tool-for-click-qpcr",
  "topic": "science",
  "section": "Science",
  "published": "2026-08-24T00:00:00.000Z",
  "source": {
    "name": "bioRxiv",
    "slug": "biorxiv",
    "url": "https://www.biorxiv.org/content/10.64898/2026.08.20.745930v1?rss=1"
  },
  "original_language": "en",
  "account": "The Click-qPCR application is a browser-based tool for relative quantitative PCR analysis. It necessitates a tidy-format CSV file with four columns: sample, group, gene, and Cq. Creating this input from qPCR instrument output usually involves manual reformatting and computation of mean Cq values for technical replicates. To streamline this procedure, researchers have created Click-Prep, an interactive web-based application tailored for generating Click-qPCR input files. Click-Prep accepts CSV, TXT, TSV, and XLS/XLSX files, allowing users to bypass instrument-generated metadata rows, interactively map columns, and manually assign experimental groups. Users can examine technical-replicate measurements, exclude rows based on established quality-control standards, and compute mean Cq values for each sample-group-target combination. The tool flags missing or nonnumeric Cq values for review and requires resolution before the mean is calculated. Additionally, Click-Prep can merge compatible formatted CSV files, such as datasets from separate qPCR plates. The final dataset is exported as a standardized CSV file containing the required four fields for Click-qPCR. By consolidating these operations into a guided browser-based workflow, Click-Prep enables users to efficiently and uniformly prepare Click-qPCR input files without the need for programming skills.",
  "summary": "Click-qPCR is a browser-based application for relative qPCR analysis that requires a tidy-format CSV file containing four columns: sample, group, gene, and Cq. Preparing this input from qPCR instrument output typically requires manual reformatting and calculation of mean Cq values for technical replicates. To simplify this process, we developed Click-Prep…",
  "key_points": [],
  "editors_take": null,
  "illustration": null,
  "coverage": {
    "outlets": 1,
    "also_reported_by": []
  },
  "ai_generated": true,
  "disclaimer": "Summaries, key points and the editor’s take are written by software from other outlets’ reporting and may contain errors — always check the linked original."
}