{
  "id": 2311595,
  "title": "A survey of multi-targeting and off-targeting sgRNAs across five genome-wide CRISPR-Cas9 knockout sgRNA libraries with GuideRefine",
  "url": "https://urgent.news/2026/08/20/a-survey-of-multi-targeting-and-off-targeting-sgrnas-across-five",
  "topic": "science",
  "section": "Science",
  "published": "2026-08-20T00:00:00.000Z",
  "source": {
    "name": "bioRxiv",
    "slug": "biorxiv",
    "url": "https://www.biorxiv.org/content/10.64898/2026.08.20.746015v1?rss=1"
  },
  "original_language": "en",
  "account": null,
  "summary": "Pooled genome-wide CRISPR-Cas9 knockout (CRISPR-KO) screening is a powerful approach for discovering new biology and identifying genetic vulnerabilities in cancers. This approach uses the Cas9 nuclease in combination with sgRNA libraries, typically consisting of 4-8 sgRNAs to induce mutations in each target gene. A critical assumption is that the effect of each sgRNA is solely due to Cas9 editing…",
  "key_points": [],
  "editors_take": null,
  "illustration": null,
  "coverage": {
    "outlets": 1,
    "also_reported_by": []
  },
  "ai_generated": true,
  "disclaimer": "Summaries, key points and the editor’s take are written by software from other outlets’ reporting and may contain errors — always check the linked original."
}