{
  "id": 14984297,
  "title": "Dynamic regulation of cystathionine gamma-lyase expression during normoxia, ischemia, and reperfusion in liver: interplay of transcription factors, microRNAs, and histone modifications",
  "url": "https://urgent.news/2026/10/16/dynamic-regulation-of-cystathionine-gamma-lyase-expression-during",
  "topic": "science",
  "section": "Science",
  "published": "2026-10-16T00:00:00.000Z",
  "source": {
    "name": "bioRxiv",
    "slug": "biorxiv",
    "url": "https://www.biorxiv.org/content/10.64898/2026.10.15.759434v1?rss=1"
  },
  "original_language": "en",
  "account": "Cystathionine gamma-lyase (CTH) plays a crucial role in hydrogen sulfide production, with its regulation under various conditions remaining unclear. We investigated CTH expression in Huh7 hepatic cells, mouse liver, and human liver across normoxia, ischemia, and reperfusion. Our analysis revealed that the -275/+91 bp region of the CTH promoter is central to its regulation, harboring binding sites for Sp1, CREB, and NFY. Computational and experimental screening identified miR-150-5p and miR-510-5p as novel post-transcriptional regulators binding directly to the CTH 3' UTR. Histone marks (H3K9ac, H3K9me3, H3K4me3, and H3K27me3) at the CTH promoter also correlated with CTH expression, varying across basal, ischemic, and reperfusion conditions. Under ischemia, Sp1 occupancy at the CTH promoter decreases, while miR-150-5p and miR-510-5p levels rise, reducing H3K9ac, ultimately suppressing CTH expression. Conversely, reperfusion restores CTH expression by increasing Sp1 and CREB binding, lowering miR-150-5p and miR-510-5p levels, and reducing H3K27me3. Our findings highlight the significant roles of Sp1, CREB, NFY, miR-150-5p, miR-510-5p, and histone modifications in CTH regulation during basal, ischemia, and reperfusion states.",
  "summary": "Cystathionine gamma-lyase (CTH), a key enzyme in hydrogen sulfide (H2S) biosynthesis, has been studied extensively in models of oxidative stress. Dysregulated CTH has been reported in cardiovascular disease, ischemia-reperfusion (I/R) injury, aging, and xenobiotic stress, where reactive oxygen species are central to pathology. Despite this, how CTH is regulated at the molecular level under basal,…",
  "key_points": [],
  "editors_take": null,
  "illustration": null,
  "coverage": {
    "outlets": 1,
    "also_reported_by": []
  },
  "ai_generated": true,
  "disclaimer": "Summaries, key points and the editor’s take are written by software from other outlets’ reporting and may contain errors — always check the linked original."
}