{
  "id": 14306565,
  "title": "A 21-Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9-Color T-Cell Functional Assay",
  "url": "https://urgent.news/2026/10/13/a-21-color-spectral-flow-cytometry-panel-for-comprehensive",
  "topic": "science",
  "section": "Science",
  "published": "2026-10-13T00:00:00.000Z",
  "source": {
    "name": "bioRxiv",
    "slug": "biorxiv",
    "url": "https://www.biorxiv.org/content/10.64898/2026.10.13.758716v1?rss=1"
  },
  "original_language": "en",
  "account": "For rat tissue immunophenotyping, a novel two-panel approach utilizing full-spectral flow cytometry has been developed. This method incorporates a 21-color comprehensive immunophenotyping panel and a 9-color intracellular cytokine staining (ICS) panel, providing a synergistic solution to the limited fluorophore pool issue. The 21-color panel is capable of mapping innate and adaptive lineages with high resolution, making it particularly useful for profiling neutrophils, monocytes, and lymphoid organs. It can simultaneously evaluate tissue-resident macrophages, dendritic cells, T cell differentiation (including regulatory T cells), and antigen-presenting cell function in lymphoid tissues. The 9-color ICS panel, on the other hand, focuses on dissecting effector T cell activation and cytokine production in Th1, Th2, and Th17 subsets. An important feature of this technique is the reservation of the PE color, which allows for the use of antibodies and probes with PE or comparable fluorophores in conjunction with the panel, thereby increasing overall flexibility. This combined approach is designed for use in rat peripheral blood, spleen, and lymph nodes, offering a comprehensive and adaptable foundation for studying complex tissue microenvironments in research areas such as tumor immunology, rheumatology, and autoimmunity.",
  "summary": "Although rats are crucial translational research models due to their immunological homology and surgical feasibility, the scarcity of conjugated antibodies has hampered high-dimensional immune profiling. To bridge this gap, we created a synergistic two-panel method that uses full-spectral flow cytometry: a 21-color comprehensive immunophenotyping panel and a 9-color intracellular cytokine…",
  "key_points": [],
  "editors_take": null,
  "illustration": null,
  "coverage": {
    "outlets": 1,
    "also_reported_by": []
  },
  "ai_generated": true,
  "disclaimer": "Summaries, key points and the editor’s take are written by software from other outlets’ reporting and may contain errors — always check the linked original."
}