{
  "id": 14094400,
  "title": "A monoallelic immunopeptidome atlas of 10 prevalent canine DLA-88 alleles",
  "url": "https://urgent.news/2026/10/12/a-monoallelic-immunopeptidome-atlas-of-10-prevalent-canine-dla-88",
  "topic": "science",
  "section": "Science",
  "published": "2026-10-12T00:00:00.000Z",
  "source": {
    "name": "bioRxiv",
    "slug": "biorxiv",
    "url": "https://www.biorxiv.org/content/10.64898/2026.10.06.756904v1?rss=1"
  },
  "original_language": "en",
  "account": "A comprehensive atlas of naturally processed MHC class I (MHC-I)-bound peptides from 10 prevalent dog leukocyte antigen (DLA-88) alleles has been established. This research offers substantial expansion to the existing canine DLA-88 immunopeptidome landscape, which previously lacked comprehensive monoallelic ligand datasets for these common alleles. Utilizing liquid chromatography-tandem mass spectrometry, the study identified 32,702 peptides ranging in length from 7 to 15 amino acids across these 10 alleles.\n\nThe 10 DLA-88 alleles displayed typical peptide length distributions and allele-specific binding motifs, which correlated with variations in binding-groove pseudo-sequences derived from human MHC-I peptide-contact residues. The study found that ligand sharing between DLA-88 alleles was linked to binding site distance. By incorporating the newly characterized DLA-88 ligand datasets, the number of published canine DLA-I alleles with ligand-characterized reference alleles was increased by over fourfold, enhancing understanding of the alleles' peptide-binding features.\n\nThe research also revealed highly conserved antigen-processing signatures in dogs, akin to those reported in humans. As a proof-of-concept application, 54 DLA-88-bound peptides identified in the immunopeptidome analysis were selected as potential candidates for canine cancer immunotherapy. Their peptide-binding features were evaluated based on the normal tissue expression profiles of their source proteins. This DLA-88 immunopeptidome atlas provides valuable insights into allele-dependent differences in antigen presentation and offers a valuable resource for developing accurate DLA-88-binding peptide prediction tools, as well as supporting peptide-based immunological studies in dogs.",
  "summary": "Characterization of naturally processed MHC class I (MHC-I)-bound peptides is essential for defining allele-specific peptide-binding features and understanding the functional consequences of MHC-I polymorphism. However, immunopeptidome analyses of dog leukocyte antigen (DLA) class I (DLA-I) molecules remain limited and comprehensive monoallelic ligand datasets for prevalent DLA-88 alleles are…",
  "key_points": [],
  "editors_take": null,
  "illustration": null,
  "coverage": {
    "outlets": 1,
    "also_reported_by": []
  },
  "ai_generated": true,
  "disclaimer": "Summaries, key points and the editor’s take are written by software from other outlets’ reporting and may contain errors — always check the linked original."
}