{
  "id": 10734437,
  "title": "Cytotoxic Effects of Multiple Pesticides and their Mixtures on Caco-2 Cells Evaluated by Using MTT and Trypan Blue Assays",
  "url": "https://urgent.news/2026/09/29/cytotoxic-effects-of-multiple-pesticides-and-their-mixtures-on-caco-2",
  "topic": "science",
  "section": "Science",
  "published": "2026-09-29T00:00:00.000Z",
  "source": {
    "name": "bioRxiv",
    "slug": "biorxiv",
    "url": "https://www.biorxiv.org/content/10.64898/2026.09.23.753704v1?rss=1"
  },
  "original_language": "en",
  "account": "Concerns have been raised regarding the potential health risks posed by pesticides via dietary and environmental exposure. This investigation aimed to assess the in vitro cytotoxicity of ten widely-used pesticides and their mixtures on human intestinal cells. The MTT assay, a measure of metabolic activity, and the trypan blue exclusion test, an indicator of cell membrane integrity, were employed to evaluate cell viability.\n\nThe findings revealed that high concentrations of all pesticides significantly diminished cell viability and vitality. Glyphosate and tebuconazole demonstrated significant toxicity at lower concentrations, specifically 0.1 mg/L and 10 mg/L, respectively. When pesticides were combined, the effects observed for individual compounds were maintained at the Top 3 and Top 8 mixtures, indicating additive (non-synergistic) toxic effects.\n\nIn conclusion, these results suggest that certain pesticide-based herbicides can induce cytotoxic effects on intestinal cells at relatively low concentrations, underscoring the necessity of utilizing a component-based approach for mixture risk assessment in humans. This research was conducted as part of the EU SPRINT project, focusing on a global health approach to sustainable plant protection.",
  "summary": "BACKGROUND: Pesticides are extensively used in agriculture, raising concerns about their potential impact on human health through dietary and environmental exposure. OBJECTIVES: This study evaluated the in vitro cytotoxicity of ten commonly used pesticides and their mixtures (lambda-cyhalothrin, cypermethrin, deltamethrin, tebuconazole, glyphosate, acetamiprid, cyprodinil, piperonyl butoxide,…",
  "key_points": [],
  "editors_take": null,
  "illustration": null,
  "coverage": {
    "outlets": 1,
    "also_reported_by": []
  },
  "ai_generated": true,
  "disclaimer": "Summaries, key points and the editor’s take are written by software from other outlets’ reporting and may contain errors — always check the linked original."
}